Understanding HPLC Verification: How We Confirm Purity

High-performance liquid chromatography (HPLC) is the backbone of our purity verification process. Every production batch is sampled and analyzed using reverse-phase HPLC to separate the target compound from synthesis byproducts, residual solvents, and degradation products.

The resulting chromatogram shows a series of peaks, each corresponding to a different compound in the sample. The area under the primary peak — relative to the total area of all peaks — gives us the purity percentage published on each Certificate of Analysis.

In addition to HPLC, batches undergo mass spectrometry to confirm molecular identity. This ensures that the compound detected isn't just 'pure' in a chromatographic sense, but is actually the correct molecule with the expected molecular weight.

Together, these two methods give researchers confidence that what's on the label matches what's in the vial — both in terms of identity and purity. Every CoA includes the chromatogram trace, purity percentage, and mass spec confirmation for full transparency.

If you'd like to review the raw data for a specific batch, search the batch number on our Lab Reports page to access the full Certificate of Analysis.